raw 264 7 macrophages  (ATCC)

Journal: Materials Today Bio

Article Title: Antithrombotic and antibacterial surface coating based on spiky silver nanoparticles: A counterattack against clotting and biofilm

doi: 10.1016/j.mtbio.2026.102762

Figure Lengend Snippet: In vitro biocompatibility of surface coatings. (A) ROS generation levels of macrophages cultured on the surface coatings. (B) Proliferation of SVEC4-10 and RAW 264.7 cells on the surfaces and (C) representative images illustrating cell morphology using LIVE/DEAD™ reagents. (D) Images and (E) haemolytic activity of surface coatings. Statistical comparisons of samples with the control were performed using one-way and two-way ANOVA tests.

Article Snippet: The cytocompatibility of the AgIONPs coatings was assessed using the LIVE/DEADTM cell viability assay, employing SVEC4-10 endothelial cells (CRL-2181, passage 10) and RAW 264.7 macrophages (ATCC TIB-71, passage 21) as representative in vitro models.

Techniques: In Vitro, Cell Culture, Activity Assay, Control

Journal: Materials Today Bio

Article Title: Antithrombotic and antibacterial surface coating based on spiky silver nanoparticles: A counterattack against clotting and biofilm

doi: 10.1016/j.mtbio.2026.102762

Figure Lengend Snippet: In vitro biocompatibility of surface coatings. (A) ROS generation levels of macrophages cultured on the surface coatings. (B) Proliferation of SVEC4-10 and RAW 264.7 cells on the surfaces and (C) representative images illustrating cell morphology using LIVE/DEAD™ reagents. (D) Images and (E) haemolytic activity of surface coatings. Statistical comparisons of samples with the control were performed using one-way and two-way ANOVA tests.

Article Snippet: Mouse endothelial cells (SVEC4-10, ATCC CRL-2181, passages 10) and mouse macrophages (RAW 264.7, ATCC TIB-71, passage 20) were cultured in low-glucose Dulbecco's Modified Eagle's Medium (DMEM, Thermo Fisher Scientific Inc., Massachusetts, USA) supplemented with 10 % fetal bovine serum and 1 % penicillin/streptomycin.

Techniques: In Vitro, Cell Culture, Activity Assay, Control

Journal: Materials Today Bio

Article Title: Antithrombotic and antibacterial surface coating based on spiky silver nanoparticles: A counterattack against clotting and biofilm

doi: 10.1016/j.mtbio.2026.102762

Figure Lengend Snippet: In vitro biocompatibility of surface coatings. (A) ROS generation levels of macrophages cultured on the surface coatings. (B) Proliferation of SVEC4-10 and RAW 264.7 cells on the surfaces and (C) representative images illustrating cell morphology using LIVE/DEAD™ reagents. (D) Images and (E) haemolytic activity of surface coatings. Statistical comparisons of samples with the control were performed using one-way and two-way ANOVA tests.

Article Snippet: The cytocompatibility of the AgIONPs coatings was assessed using the LIVE/DEADTM cell viability assay, employing SVEC4-10 endothelial cells (CRL-2181, passage 10) and RAW 264.7 macrophages (ATCC TIB-71, passage 21) as representative in vitro models.

Techniques: In Vitro, Cell Culture, Activity Assay, Control

Journal: Biotechnology Reports

Article Title: Insulin Resistance, Anti-inflammatory, and Antioxidant In vitro Activity of Heterologously Expressed Arenin from Dryophytes arenicolor

doi: 10.1016/j.btre.2026.e00947

Figure Lengend Snippet: Anti-inflammatory potential of arenin on Raw 264.7 cells . After arenin incubation, nitric oxide production was stimulated with LPS (1 μg/mL). Untreated cells were used as the control group. a-h Different letters indicate statistical differences in the interaction between concentrations and lipopolysaccharide (LPS) stimulus, analyzed using the Tukey test ( p < 0.05).

Article Snippet: Human hepatocyte carcinoma (HepG2) cells, human primary dermal fibroblasts (HDFa) cells, and murine macrophage (RAW 264.7) cells were obtained from the American Type Culture Collection (ATCC, VA, USA).

Techniques: Incubation, Control

Journal: APL Bioengineering

Article Title: Synthetic mucus biomaterials enable localized therapeutic antibody delivery in inflammatory bowel disease

doi: 10.1063/5.0297897

Figure Lengend Snippet: Immunomodulatory function of mucins (PSIM) and synthetic mucus (SM) biomaterials on macrophages. (a) Fluorescence imaging of pHrodo bioparticle uptake by RAW 264.7 macrophages. Macrophages were stained with phalloidin (red) and DAPI (blue) to visualize actin and nuclei, respectively ( n = 4 technical replicates). Scale bar represents 50 μ m. (b) The percentage of macrophages that phagocytized pHrodo bioparticles after treatment was quantified via flow cytometry ( n = 4 technical replicates). (c) The mean fluorescence intensity (MFI) was measured using flow cytometry to determine the phagocytic capacity of macrophages that phagocytize pHrodo bioparticles ( n = 4 technical replicates). non-significant (ns), * P < 0.05, *** P < 0.001, and **** P < 0.0001 for one-way ANOVA. Data show mean ± SD. (d) Graphical depiction of the study to examine impact of direct treatment with mucins in solution and hydrogel form on macrophage phenotype. (e)–(j) Flow cytometry analysis of cell surface markers, CD80, and CD206 in unstimulated macrophages ( n = 4 technical replicates). (k)–(n) Cytokine production (TNF-α, IL-6, TGF-β, IL-10) in unstimulated macrophages determined by ELISA ( n = 4). (o) Graphical depiction of the study to examine the impact of direct treatment with mucins in solution and hydrogels on LPS-stimulated macrophage phenotype. (p)–(u) Flow cytometry analysis of cell surface markers, CD80 and CD206, in LPS-stimulated macrophages ( n = 5–8 technical replicates). (v)–(y) Cytokine production (TNF-α, IL-6, TGF-β, IL-10) in LPS-stimulated macrophages determined by ELISA ( n = 5–8 technical replicates). Datasets in (e)–(j) and (p)–(u) were statistically analyzed via Welch's t-test: * P < 0.05, ** P < 0.01, *** P < 0.001, and **** P < 0.0001. Datasets in (k)–(n) and (v)–(y) were statistically analyzed via one-way ANOVA: * P < 0.05, *** P < 0.001, and **** P < 0.0001.

Article Snippet: Murine RAW 264.7 macrophages (ATCC TIB-71) were cultured in DMEM media with 10% fetal bovine serum and 1% Penicillin-Streptomycin solution at 37 °C and 5% CO 2 .

Techniques: Fluorescence, Imaging, Staining, Flow Cytometry, Enzyme-linked Immunosorbent Assay

Journal: APL Bioengineering

Article Title: Synthetic mucus biomaterials enable localized therapeutic antibody delivery in inflammatory bowel disease

doi: 10.1063/5.0297897

Figure Lengend Snippet: In vitro efficacy of anti-TNF-α%-loaded synthetic mucus gels. Production of (a) TNF-α (n = 6 technical replicates), (b) IL-10 (n = 6 technical replicates), and (c) surface marker expression (CD80/CD206) (n = 3 technical replicates) in LPS-stimulated RAW 264.7 mouse macrophages following treatment with synthetic mucus gels, * p <0.05, ** P < 0.01, **** p <0.0001 for one-way ANOVA with Tukey's multiple comparison test.

Article Snippet: Murine RAW 264.7 macrophages (ATCC TIB-71) were cultured in DMEM media with 10% fetal bovine serum and 1% Penicillin-Streptomycin solution at 37 °C and 5% CO 2 .

Techniques: In Vitro, Marker, Expressing, Comparison